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Japanese Researchers Regenerate Joints in Amputated Frog Limbs

Researchers at Kyoto University, Japan, have regenerated functional joints in frogs by activating a newly discovered reintegration mechanism. Further understanding of this process may help transplanted tissues integrate with the original organs and limbs after surgical removal or amputation. The study was published January 6 in the open access journal Regeneration.

“We expect that by applying this approach to other animals, we may also achieve functional joint regeneration in mammals, including humans, in the future,” said study author Kiyokazu Agata, PhD, a professor at Kyoto University and group director of the university’s Laboratory for Evolutionary Regeneration Biology.

Some animals, such as newts and salamanders, can regenerate a smaller, but fully functioning limb after the original has become detached. Frogs are considered to be the intermediate between such animals and mammals that cannot regenerate limbs. Frogs regenerate “spikes,” which are cartilage rods protruding from the amputation area. These new cartilage limbs lack joints, and are therefore unable to bend at will. A functional joint requires integration of multiple tissues: two opposing skeletal elements forming an interlocking structure, and muscles that insert into skeletal tissues via tendons across joints. Newts, but not frogs, are capable of reconnecting these tissues.

When a frog limb is detached at an elbow joint, however, the interaction between the remaining and the regenerated tissues affects the regrowth of the regenerated elbow so that it resembles the original tissue more closely. As a result, the tissue attached to the joint is more similar in size to the original limb. The team named this interaction mechanism “reintegration.”

“As a next step we would like to attempt functional joint regeneration in mice by activating the reintegration mechanism,” said Agata.

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